Home Contents up to 2015 Editors Instructions to authors Subscription Letters

Zb. Bioteh. Fak. Univ. Ljubl., Kmet. Zooteh., 78(December 2001)2, 85–103.

MAPPING OF CONTENTS IN "ZBORNIK BIOTEHNIŠKE FAKULTETE UNIVERZE V LJUBLJANI. KMETIJSTVO. ZOOTEHNIKA" (RESEARCH REPORTS BIOTECHNICAL FACULTY UNIVERSITY OF LJUBLJANA. AGRICULTURE. ZOOTECHNY): AN ANALYSIS OF NARROWER AND BROADER SUBJECT AREAS

a)
a)
Univ. of Ljubljana, Biotechnical Fac., Agronomy Dept., Jamnikarjeva 101, SI-1000 Ljubljana, Slovenia, Ass.Prof., Ph.D.

Original scientific paper
Language: English

ABSTRACT
A bibliometric analysis of volumes 74 (1999) and 76 (2000) (altogether 48 articles) of Zbornik Biotehniške fakultete Univerze v Ljubljani. Kmetijstvo. Zootehnika (Research Reports Biotechnical Faculty University of Ljubljana. Agriculture. Zootechny) was based on the Agrovoc descriptors (de) assigned by an indexer (ENI), computer assigned DE (ENC) and category codes (CC) assigned as indexing terms to the respective articles in the Agris database. Two subject indexes based on ENI and ENC coupled together, and on CC were constructed as a material for further analysis. The scatter of indexing terms was then compared with some scientometric laws such as those by Bradford, Zipf, and others. Among 357 different DE (ENI and ENC coupled together in one file) some 80 % were assigned only once or twice. 210 DE occured only once. Organism DE maintained the highest frequency (bacteria: 16 times, followed by useful animals: 13 times). Some 20 % of all different DE were assigned to half of the articles. Among ENI 154 of 221 terms occured only once. Aspect DE possessed the highest frequency among ENI (chemical composition: 11 times, followed by proximate composition: 10 times). Some indexing characteristics were further highlighted such as the use of synonyms and related terms. The results of macroindexing with CC were interpreted with some limitations as there are in classification schemes no sufficient macrocategories referring to microbiology or biotechnology of microorganisms. All of the records of the Slovenian Agris output were processed by one indexer thus maintaining a relatively high indexing consistency what facilitates better information retrieval.

Key words: animal sciences / zootechny / journals / indexing / indexes / thesauri / identification / data collection / data analysis / data processing / information science / information services / documentation / databases


Zb. Bioteh. Fak. Univ. Ljubl., Kmet. Zooteh., 78(December 2001)2, 105–115.

QUANTITY ANALYSIS OF SWEET CHESTNUT (Castanea sativa Mill.) POLLEN GRAINS IN HONEYS HARVESTED IN SLOVENIA IN 1999

Iztok BERNARD and a)
a)
Agricultural Institute of Slovenia, Hacquetova 17, SI-1001 Ljubljana, Slovenia, Ass.Prof., Ph.D.

Original scientific paper
Language: Slovene

ABSTRACT
Honey samples, 129 in total, were randomly sampled in Slovenia in 1999. Pollen structure of honey samples was analysed, 44 different types of pollen grains were determined. Pollen grains of sweet chestnut are present in 94.6 % of samples. On average, their content was of 59.98 % per sample. 42 honey samples contain more than 90 % of such pollen, and could be described as pure sweet chestnut honey. Sweet chestnut pollen content is typical of honey produced in Slovenia and could be used as main criterion to distinguish Slovenian honey from honey produced in the other middle European countries situated northern of Slovenia.

Key words: honey bees / honey / sweet chestnut / pollen analysis / Slovenia

____________________
* The paper is a part of graduation thesis (justification February 20, 2001), supervisor ass.prof. Janez Poklukar, Ph.D.

Zb. Bioteh. Fak. Univ. Ljubl., Kmet. Zooteh., 78(December 2001)2, 117–126.

STATISTICAL ANALYSIS OF AN EXPERIMENT WITH TIME COMPONENT *

Katarina KOŠMELJ a), Andrej BLEJEC and Drago KOMPAN
a)
Univ. of Ljubljana, Biotechnical Fac., Agronomy Dept., Jamnikarjeva 101, SI-1000 Ljubljana, Slovenia, Prof., Ph.D.

Preliminary report
Language: Slovene

ABSTRACT
In this paper we present the statistical analysis which we used to assess an experiment undertaken at the Zootechnical Department, Biotechnical Faculty, University of Ljubljana. Due to the type of data, standard statistical methods can not be used. We present our ideas and statistical approaches that give the required answers. The objective of this paper is to present the statistical analysis to the public since this type of experiment is quite common in biotechnical domain.

Key words: statistics / statistical analysis / time series / data transformation / data standardization / analysis of variance / Wilcoxon test / goats / somatic cells


Zb. Bioteh. Fak. Univ. Ljubl., Kmet. Zooteh., 78(December 2001)2, 127–135.

DETECTION OF ANTIBODIES TO PATHOGENIC MICROORGANISMS IN THE CHICKEN EMBRYO'S FLUIDS *

Darja HERMAN, a) and Dušan BENČINA
a) Univ. of Ljubljana, Biotechnical Fac., Zootechnical Dept., Groblje 3, SI-1230 Domžale, Slovenia, Ass.Prof., Ph.D., M.Sc.

Original scientific paper
Language: Slovene

ABSTRACT
Certain avian pathogens can be transmitted in chickens via hatching eggs. Specific maternal antibodies appearing in the egg yolk and egg white are able to protect embryo against a variety of vertically transmitted bacterial and viral pathogens. Immunoenzyme tests using different monoclonal antibodies (mAb) to chicken immunoglobulins G (IgG), IgA and IgM were used for detection of antibodies to Mycoplasma synoviae, M. gallisepticum, Ornithobacterium rhinotracheale and Newcastle disease virus in the allantoic fluid (ALF), amniotic fluid (AMF), bile and serum of chicken embryos on different stages of the embryonic development. In the ALF of 7-day-old embryos IgG antibodies to M. synoviae were demonstrated for the first time in this study. Certain samples contained also IgG antibodies to M. gallisepticum and Newcastle disease virus. In addition, IgA, and IgM were also found in the ALF. It seems that transfer of IgA and IgM from the egg white to ALF is selective. In AMF specific IgG antibodies to M. synoviae were detected on day 7 of the embrionic development. From 12th day of the embryogenesis onward levels of IgA, IgG and IgM in AMF were apparently increased. Specific IgA and IgG antibodies to M. synoviae and O. rhinotracheale were detected in bile of 13-day-old embryos. In the blood, which was taken from the chorioallantoic veins from the 13th to 16th day of the embryogenesis, IgG and IgA were found.

Key words: poultry / chicken embryo / immunology / immunoglobulins / allantoic fluid / amniotic fluid / bile

____________________
* The paper is a part of graduation thesis (justification September 20, 2001), supervisor ass.prof. Mojca Narat, Ph.D., co-supervisor Dušan Benčina, Ph.D.

Zb. Bioteh. Fak. Univ. Ljubl., Kmet. Zooteh., 78(December 2001)2, 137–149.

ESTIMATION OF ENERGY VALUE IN HAY *

a), Jože VERBIČ and Tomaž ŽNIDARŠIČ
a) Agricultural Institute of Slovenia, Hacquetova 17, SI-1001 Ljubljana, Slovenia, research adviser, Ph.D., M.Sc.

Original scientific paper
Language: Slovene

ABSTRACT
The concentration of net energy for lactation (NEL) of 100 hay samples that were collected in 1999 and 2000 was estimated by the use of in vitro method, which was based on the gas produced during the incubation of samples with rumen liquor. Values were compared to the estimates obtained by the procedures which are usual for Slovenian laboratories, i.e. on the basis of chemical composition and digestibility coefficients from tables or by the use of regression equations from literature. It was established that NEL concentrations that were estimated on the basis of DLG (1982) tables deviated from in vitro estimated values for 0.22 ± 0.35 MJ kg–1 dry matter (DM). Estimates on the basis of recently published tables and equations deviated from in vitro estimated values for 0.25 ± 0.31 MJ (DLG, 1991), 0.20 ± 0.33 MJ (DLG, 1997) and 0.16 ± 0.33 MJ (GfE, 1998) kg–1 DM. The equation according to Stekar et al. (1994) deviated on average from in vitro values for 0.01 ± 0.34. Regression analysis showed that all estimates deviated systematically from in vitro estimated values. For the estimation of low quality samples from extensively managed grasslands which contain less than 5.2 MJ NEL kg–1 DM the usage of old German tables (DLG, 1982) can be recommended while for samples which contain more than 5.2 MJ NEL kg–1 DM recently published tables (DLG, 1997) and official German equations (GfE, 1998) are more suitable. For average samples from Slovenian farms the highest accuracy can be achieved by the use of the equation proposed by Stekar et al. (1994). Relatively high average individual deviations from in vitro estimated values (0.27–0.32 MJ NEL kg–1 DM) were characteristic for all estimates. Maximal individual deviations exceeded 1 MJ NEL kg–1 DM. New regression equations for estimation of metabolizable energy concentration and NEL were proposed. It was found out that by the use of new equations deviations from the in vitro estimated values can be reduced by 10 to 50 %. Unexplained deviations of about 0.2 MJ NEL kg–1 DM cannot be avoided by the means of classical Weende analysis.

Key words: animal nutrition / feed / hay / chemical composition / metabolizable energy / net energy for lactation / estimation / statistics / statistical analysis / regression equation

____________________
* Part of the data presented in this paper originates from graduation thesis (justification July 5, 2001), supervisor ass. prof. Jože Verbič, Ph.D. and co-adviser ass. prof. Andrej Lavrenčič, Ph.D.

Zb. Bioteh. Fak. Univ. Ljubl., Kmet. Zooteh., 78(December 2001)2, 151–160.

DETERMINATION OF CHEMICAL COMPOSITION, IN VITRO DRY MATTER DIGESTIBILITY AND IN VITRO GAS PRODUCTION OF FORAGE BY NEAR-INFRARED REFLECTANCE SPECTROSCOPY

a), Bruno STEFANON, Barbara TOSO and Piero SUSMEL
a)
Univ. of Ljubljana, Biotechnical Fac., Zootechnical Dept., Groblje 3, SI-1230 Domžale, Slovenia, Ass.Prof., Ph.D.

Original scientific paper
Language: English

ABSTRACT
One hundred dried samples of forage, harvested three or four times during 1997 and 1999 in different morphological stages were analysed for chemical composition, in vitro dry matter digestibility (IVDMD; Tilley and Terry, 1963) and in vitro gas production (Menke and Steingass, 1988) by laboratory methods and by near infrared reflectance spectroscopy (NIRS). NIRS predicted the main chemical components, such as crude protein, crude fibre, neutral detergent fibre, acid detergent fibre, with a very high degree of accuracy (i.e. the determination coefficient (R2) varied from 93.5 % for ADF to 98,2 % for NDF), while the coefficients of determination of crude ash, ether extract and acid detergent lignin were 79.6, 85.1 and 82.3 %, respectively, with standard errors of cross validation (SECV) exceeding 9.0 %. The IVDMD was also determined with a high degree of accuracy (R2 = 94.3 %; SECV = 4.3 %), such as parameters of gas production "B" (total gas production) and "A" (constant factor describing the decay in specific gas production rate), estimated with the Gompertz model, which have R2 and SECV values of 93.0 and 3.8 % and 94.5 and 8.0 %, respectively. The parameter "C" (the specific fermentation rate) was predicted with a somewhat lower degree of accuracy (R2 = 78.3 and SECV = 9.6 %).

Keywords: animal nutrition / forage / chemical composition / in vitro dry matter digestibility / in vitro gas production / analytical chemistry / near infrared reflectance spectroscopy (NIRS)


Zb. Bioteh. Fak. Univ. Ljubl., Kmet. Zooteh., 78(December 2001)2, 161–170.

CONCENTRATION OF ACID DETERGENT FIBRE BOUND NITROGEN IN GRASSLAND FORAGE

a), Drago BABNIK and Mojca RESNIK
a)
Agricultural Institute of Slovenia, Hacquetova 17, SI-1001 Ljubljana, Slovenia, senior researcher, Ph.D., M.Sc.

Original scientific paper
Language: Slovene

ABSTRACT
The relations between the proportion of acid detergent insoluble nitrogen (ADIN, in g kg –1 total N) on one hand and concentrations of crude protein and crude fibre on the other have been investigated. It was found that with increasing concentration of crude protein (CP) the proportion of ADIN in grassland forages decreased while with increasing concentration of crude fibre (CF) it increased. At given CP concentration the proportion of ADIN in legumes was higher than in grasses. Proportion of ADIN in hay from extensively managed grasslands was considerably higher than in hay from intensively managed grasslands. At given CP concentration there was a tendency of lower proportion of ADIN in first cut silages in comparison to silages which were made from consecutive cuts. Equations which enabled prediction of the proportions of ADIN in the samples of green forage, hay and silage on the basis of concentration of CP were calculated. It was established that they allowed rough estimates. For the present, accurate concentration of ADIN can be achieved only by the means of chemical analysis.

Key words: feed / grass / silage / hay / acid detergent / insoluble nitrogen / crude proteins / crude fibre / animal nutrition / statistics / statistical analysis / equations / prediction


Zb. Bioteh. Fak. Univ. Ljubl., Kmet. Zooteh., 78(December 2001)2, 171–183.

THE INFLUENCE OF BACTERIAL INOCULANTS ON AEROBIC STABILITY OF SILAGE *

a) and Jasna M.A. STEKAR
a) Agricultural Institute of Slovenia, Hacquetova 17, SI-1001 Ljubljana, Slovenia.

Original scientific paper
Language: Slovene

ABSTRACT
The effect of bacterial inoculants on aerobic stability of silage was studied on the basis of statistical processing of data from literature. When forage had higher content of dry matter aerobic stability of grass silage, inoculated with homofermentative lactic acid bacteria, was lower than in silage without additives. When forage had low dry matter content, homofermentative lactic acid bacteria had a favourable effect on aerobic stability and quality of grass silage. Inoculation of maize, sorghum and cereals improved the quality but impaired the aerobic stability of silages. An exception was inoculant of Lactobacillus plantarum and Enterococcus faecium that slightly improved aerobic stability of cereals silage. Heterofermentative lactic acid bacteria Lactobacillus buchneri improved the quality and aerobic stability of maize, sorghum and cereal silages and was unaffected in grass silage. Additives which contained propionic acid bacteria have no effect on the fermentation of plants which are inherently unstable at aerobic exposure.

Key words: feed / corn silage / grass silage / animal nutrition / microbiology / bacterial inoculants / lactic acid bacteria / homofermentative lactic acid bacteria / heterofermentative lactic acid bacteria / propionic acid bacteria / aerobic stability

____________________
* This paper is a seminar essay, supervisor prof. Jasna M.A. Stekar, Ph.D.

Zb. Bioteh. Fak. Univ. Ljubl., Kmet. Zooteh., 78(December 2001)2, 185–195.

THE SULPHUR AND CHLORINE CONTENTS OF GRASS AND MAIZE SILAGE

Marija RAJČEVIČ a), Alojz VIDIC, Pavel KUBA, Marijan NEČEMER and Peter KUMP
a)
Poslovni sistem Mercator d.d., Dunajska 107, SI-1000 Ljubljana, Slovenia, senior scientist, Ph.D., M.Sc.

Original scientific paper
Language: English

ABSTRACT
In seven defined samples of grass and in six samples of maize silage the contents of sulphur and chlorine were determined by two analytical procedures: both elements with X-ray fluorescence spectrometry (XRF), sulphur separately with inductively coupled plasma – atomic emission spectrometry (ICP-AES) and chlorine according to Volhard (Skoog and West, 1963; Official Journal of the EC, No.L. 155/13, 12/07/71). The average contents of sulphur in grass silage, g kg–1 DM, were 2.53 g and 1.91 g, in maize 0.87 g and 0.75 g by XRF and ICP-AES, respectively. The differences in the content of sulphur between the two methods were statistically significant (P < 0.01 and P < 0.05). The average contents of chlorine in grass silage, g kg–1 DM, were 7.69 g and 9.09 g, and in maize 2.00 g and 2.55 g by XRF and Volhard, respectively. The differences between the two methods were statistically significant (P < 0.01).

Key words: feed / grass silage / maize silage / sulphur / chlorine / animal nutrition / analytical chemistry / X-ray fluorescence spectrometry / atomic emission spectrometry / titration


Zb. Bioteh. Fak. Univ. Ljubl., Kmet. Zooteh., 78(December 2001)2, 197–209.

CONNECTION AMONG CAROTENOIDS IN MAIZE SILAGE AND YOLK COLOUR *

Eva TKALČIČ a), Jasna M.A. STEKAR and Antonija HOLCMAN
a)
Kmetijsko gozdarski zavod Maribor, Vinarska 14, SI-2000 Maribor, Slovenija, M.Sc.

Original scientific paper
Language: Slovene

ABSTRACT
In the feeding trial with 600 laying hens of Isa Brown the effect of ensiled maize grains on colour of yolk and content of beta carotene and xantophylls was studied. At the age of 32 weeks the hens were divided into six groups, each contained 100 animals. The experiment lasted 154 days. Two maize hybrids Raissa and Lotus were compared. In the ration there were 40 % and 60 % of dry matter from the ensiled grains respectively. The supplemental feeding mixture was the same in both cases. Groups on trial rations and one control group on feeding mixture with no added pigment were fed restrictively. In the second control group the laying hens were fed on complete feeding mixture ad libitum. The results were statistically processed by SAS/STAT (SAS Institute Inc., 1989). Rations with 60 % of silage caused significantly darker colour of yolk. The amount of xanthophylls in the silage was significantly related to xantophylls in yolk. The content of xanthophylls in yolk depended on their content in rations. The maize hybrid Raissa affected the darker of yolk colour. Lotus increased the content of beta-carotene in yolk. There were no differences in the content of beta-carotene between the hybrids while Lotus contained significantly less xanthophylls.

Key words: animal nutrition / feed / silage / maize grains / hybrids / beta carotene / xantophylls / eggs / yolk / colour

____________________
* The article is a part of the master of science thesis (justification February 22, 2001), supervisor prof. Jasna Stekar, Ph.D., co-advisor ass.prof. Antonija Holcman, Ph.D.

Zb. Bioteh. Fak. Univ. Ljubl., Kmet. Zooteh., 78(December 2001)2, 211–218.

THE EFFECT OF DIET CONTAINING ARSENIC (III) OXIDE ON THE TRAITS OF EGGS *

a), Špela MALOVRH and Vanja KNEZ
a)
Univ. of Ljubljana, Biotechnical Fac., Zootechnical Dept., Groblje 3, SI-1230 Domžale, Slovenia, Ass.Prof., Ph.D., M.Sc.

Original scientific paper
Language: English

ABSTRACT
The main objective of this study was to determine the effect of different arsenic concentration in feed on the physical traits of eggs. Twenty-four Rhode Island Red laying hens were divided into four equal groups. Three groups received feed mixture with additional arsenic in the form of arsenic (III) oxide in the following concentrations: 7.5 mg kg–1 (group 1), 15.0 mg kg–1 (group 2) and 30.0 mg kg–1 (group 3). The control group was fed on diet without additional arsenic. From the third to the seventh day eggs were collected and measured for physical traits. It has been established that during the trial period arsenic concentration in diet had a highly significant effect (P = 0.0001) on shell colour, egg mass, height of dense albumen, as well as on the number of Haugh units. The effect on shell mass and shell thickness was significant (P £ 0.01), while there was no significant influence on the colour of yolk. Changes in physical traits of eggs were not significant during this period. The only exception was the change in yolk colour, yet it was not due to arsenic, but to feeding mixture with added colouring the hens had been fed on prior to trial.

Key words: poultry / hens / animal nutrition / feed / arsenic / eggs / mass / height of dense albumen / Haugh units / eggshell / yolk / colour

____________________
* The paper is a part of graduation thesis (justification May 30, 1995), supervisor ass.prof. Antonija Holcman, Ph.D., co-advisor assoc.prof. Miroslav Štruklec, Ph.D.

Zb. Bioteh. Fak. Univ. Ljubl., Kmet. Zooteh., 78(December 2001)2, 219–227.

TWO TRAITS MODEL FOR LITTER SIZE OVER PARITIES IN PIGS *

a)+ and Milena KOVAČ
a)
Univ. of Ljubljana, Biotechnical Fac., Zootechnical Dept., Groblje 3, SI-1230 Domžale, Slovenia,  M.Sc.
+ Present address: Agricultural Institute of Slovenia, Hacquetova 17, SI-1001 Ljubljana, Slovenia

Original scientific paper
Language: Slovene

ABSTRACT
The aim of the study was to estimate genetic correlations between litter size in gilts and sows for number of born and number of liveborn piglets. Total 24 334 litters of Swedish Landrace (SL), Large White (LW), and both F1 sows; line 12 (SL × LW) and line 21 (LW × SL) were included. High genetic correlations for number of born piglets (0.92) and also for number of liveborn piglets (0.92) were obtained by animal model. Due to high genetic correlations litter size across litters from 1st to 6th parity can be treated as the same trait. The estimates of heritability for litter size in gilts and sows were higher for number of born (0.14) than for number of liveborn piglets (0.12). The results confirmed the adequacy of a simple repeatability model in the selection for litter size in the population analyzed.

Key words: pigs / reproduction / litter size / statistics / statistical analysis / selection / genetic correlations / animal model / two traits model / repeatability model

____________________
* This paper is a part of a master of science thesis (justification 19.4.2000), supervisor assoc. prof. Milena Kovač, Ph.D.

Zb. Bioteh. Fak. Univ. Ljubl., Kmet. Zooteh., 78(December 2001)2, 229–242.

EQUATIONS FOR ASSESSMENT OF LEAN MEAT CONTENT IN PIG CARCASSES ON SLAUGHTER LINE

Špela MALOVRH a), Milena KOVAČ, Marjeta ČANDEK POTOKAR, Silvester ŽGUR and Blaž ŠEGULA
a)
Univ. of Ljubljana, Biotechnical Fac., Zootechnical Dept., Groblje 3, SI-1230 Domžale, Slovenia,  Ass.

Original scientific paper
Language: Slovene

ABSTRACT
Assessment of meatiness on slaughter line based upon measurements of explanatory variables and equation for lean meat content in which measurements are entered. Equation is evaluated using data from preceding dissection of sufficient number of carcasses. Considering EU legislation for computation of new formula, EU-reference and partial dissection were used, as well as double-regression as a statistical method. Sixty-one carcasses were dissected according to EU-reference method, and the sample for partial dissection included 130 carcasses. Equations for lean meat content for method with apparatus Hennessy Grading Probe (HGP) and two-point method were computed. Equation for HGP met criterion for residual standard deviation (RSD) of 2.5 (2.43), so it is convenient for introduction of HGP method on the slaughter line. Substitutive two-point method did not reach 2.5 for RSD. Nevertheless, current equation DM5 for two-point method still sufficiently estimate the lean meat content. Prior to the introduction of the new method for HGP on slaughter line, verification is needed.

Key words: pigs / lean meat content / double-regression / Slovenia

 

University of Ljubljana Biotechnical Faculty