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Acta agriculturae Slovenica, 88(November 2006)1, 3–10.

Agris category codes: L53
COBISS Code           1.01
Language: Slovene

ABSOLUTE AND RELATIVE FECUNDITY, EGG WEIGHT AND INFLUENCE OF EGG WEIGHT ON BODY WEIGHT OF LARVAE AND ALEVINS IN RAINBOW TROUT (Onchorynchus mykiss) AT FIRST AND SECOND SPAWNING

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a)

a) Univ. of Ljubljana, Biotechnical fac., Zootechnical Dept., Groblje 3, SI-1230 Domžale, Slovenia, Assoc.Prof., Ph.D., M.Sc.

ABSTRACT

From a certain number of females, eggs were collected during their first and second spawning. Body mass at spawning (MPlm) and absolute fecundity (PAbs) was registered for each female. On the basis of these data the relative fecundity was calculated (PRel). For each female 50 eyed eggs as well as 50 offspring at different stages of growth were individually weighted. Weightings were done 11 days (T1), 6 weeks (T2) and 10 weeks (T3) after hatching. By such procedure mean weight of the eggs (MIker) and the mean body mass of alevins attained at T1, T2 in T3 (MRib1, MRib2 and MRib3) were obtained. The statistical model of partial regression including PRel and MIker as independent variables was used to evaluate the influence of MIker on MRib1, MRib2 and MRib3. Results indicate that from heavier eggs heavier larvae are hatched. Nevertheless, the influence of egg weight on body mass is only short termed. Partial regression coefficient of MIker on MRib3 is not statistically significant regardless whether relation between eggs and alevins at first or second spawning is observed. According to our results it is suggested that eggs collected at first spawning should not be discarded even they appear to be too small for production. At age of 10 weeks after hatching, average size of alevins hatched from small eggs collected at first spawning did not to essentially differ from average size of alevins hatched from large eggs collected at second spawning

Key words: aquaculture / fish / trouts / rainbow trout / Onchorynchus mykiss / reproduction / eggs / size / larvae / mass
Acta agriculturae Slovenica, 88(November 2006)1, 11–18.

Agris category codes: L10
COBISS Code           1.01
Language: English

Effect of PPARGC-1 gene on backfat thickness in pigs [1]

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a), , and

a) Univ. of Ljubljana, Biotechnical fac., Zootechnical Dept., Groblje 3, SI-1230 Domžale, Slovenia.

ABSTRACT

PPARGC-1 gene is assumed to be a candidate gene with a major effect on fatness and meat quality. In this study, frequency of T/A substitution at position 1378 in PPARGC-1 gene in pigs was examined in boars of 4 different breeds. Frequency of genotypes and alleles were compared between animals with the thinnest and with the thickest backfat. Differences in genotype frequency between groups were significant in dam line of Slovenian Landrace (SL11) and sire line of Large White breed (LW66). Allele A was predominantly present in animals with thick backfat in SL11 (71.05%), LW66 (58%) and in sire line of Slovenian Landrace (67.65%). Differences in allele frequency were significant in SL11. We found a significant effect on phenotypic and breeding values for backfat in population of Large White breeds, where homozygotes TT had the thickest backfat, and heterozygotes the thinnest. In population of Slovenian Landrace breed the thickest backfat had homozygotes AA and the thinnest homozygotes TT. Similar results were obtained by analysis of gene effect on breeding values. Inconsistency could be explained by different background of allele, epistasis and intensity of selection.

Key words: pigs / backfat / genetics / PPARGC-1 gene / polymorphism
 

[1] This article is part of a graduation thesis ´Effect of polymorphism T1378A in PPARGC-1 gene on fattening and growth rate traits in pigs´, issued by Tina Flisar, supervisor prof. Peter Dovč, Ph.D.


Acta agriculturae Slovenica, 88(November 2006)1, 19–27.

Agris category codes: L01, Q04
COBISS Code           1.01
Language: Slovene

THE INFLUENCE OF PIG FODDER COMPOSITION ON QUALITY OF DRIED PORK NECK [1]

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a), Tomaž POLAK, Lea GAŠPERLIN, Sergeja VIDAKOVIČ and
Božidar ŽLENDER

a) Univ. of Ljubljana, Biotechnical fac., Zootechnical Dept., Groblje 3, SI-1230 Domžale, Slovenia.

ABSTRACT

The aim of the article is to show the influence of diet composition and sex on sensory and instrumental quality of dried pork neck. For the research we used 12 hogs and 12 sows. They were separated into 4 groups regarding different food mixture (flaxseed, rapeseed, rapeseed+vit.E and control mixture). All 24 samples of dried neck were analyzed on fat content. Sensory analysis of dried necks and instrumental measurement of colour (CIE -L, -a, -b) and texture (Kramer shear cell) were performed. The results showed that different fodder composition had no influence on fattiness of the product, while sex had a significant influence. Aftertaste was perceived by feeding with addition of flaxseed and rapeseed. Added vitamin E decreased sensory characteristics (smell, aroma, texture) and gave lighter colour. The firmer texture was shown by feeding with rapeseed. Neck from hogs had darker colour and firmer texture than neck from sows.

Key words: pigs / animal nutrition / feed / feed additives / sex / meat / dried pork neck / quality / sensory properties / instrumental properties
 

[1] This article is part of a graduation thesis ´The influence of pig fodder composition on quality of dried neck´, issued by Marjeta Furman, supervisor prof. Božidar Žlender, Ph.D.


Acta agriculturae Slovenica, 88(November 2006)1, 29–36.

Agris category codes: L50
COBISS Code           1.01
Language: English

MHS status and salivary cortisol concentration in individually housed pigs [1]

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a), and Ivan ŠTUHEC

a) Univ. of Ljubljana, Biotechnical fac., Zootechnical Dept., Groblje 3, SI-1230 Domžale, Slovenia, Ph.D., B.Sc.

ABSTRACT

Salivary cortisol was evaluated as stress measure in pigs of two malignant hyperthermia (MH) genotypes (NN and Nn), housed individually in metabolic cages and in comfortable large pens with straw. Three replicates were done, each including 8 German Landrace barrows, four (2 NN, 2 Nn) housed in pens and four (2 NN, 2 Nn) in metabolic cages. Altogether there were 24 animals included in the experiment. Saliva samples of all animals were collected simultaneously every 15 minutes between 8.00 and 11.00 a.m. on days 8, 22 and 36 of the experiment. Pigs in more stressful conditions (metabolic cages) had higher salivary cortisol values than pigs in pens, indicating that salivary cortisol might be a suitable indicator of stress in pigs. Higher salivary cortisol values in NN- in comparison to Nn- pigs indicated stronger response to stressful conditions in NN-genotype.

Key words: pigs / animal physiology / endocrinology / malignant hyperthermia / saliva / plasma / cortisol
 

[1] The paper presents a part of the results from the Ph.D. thesis ´The estimation of the degree of stress in pigs with the evaluation of cortisol concentration in plasma, urine and saliva´, issued by Nataša Siard, supervisor prof. Ivan Štuhec, Ph.D.


Acta agriculturae Slovenica, 88(November 2006)1, 37–51.

Agris category codes: Q03
COBISS Code           1.01
Language: English

THE Presence of some pathogen micro organisms, yeasts and moulds in cheese samples produced at SMALL DAIRY-PROCESSING PLANTS

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a) and Slavica GOLC TEGER

a) Univ. of Ljubljana, Biotechnical fac., Zootechnical Dept., Groblje 3, SI-1230 Domžale, Slovenia, Ass.Prof., Ph.D., M.Sc.

ABSTRACT

The presence of pathogenic and some indicator micro organisms was studied in 40 samples of cheese comprising 14 curd samples, 13 samples of soft ripened salted or non-salted cheese and 13 samples of semi-hard cheese manufactured at five small dairy-processing plants. The mean number of coagulase-positive staphylococci in all tested samples was 2.5 × 104 cfu[1] g1, while the number of E. coli bacteria was 1.4 × 106 cfu g1. In 20.0% out of 40 samples tested, the number of coagulase-positive staphylococci exceeded the prescribed regulations, particularly in soft cheese (12.5%) and curd (7.5%). About 17.5% of samples were contaminated with E. coli in higher concentrations than national valid regulations allowed. The number of E. coli was mostly exceeded in soft cheese and curd in 12.5% and 5.0% of all examined samples, respectively. One sample of semi hard cheese was contaminated with sulphite-reducing clostridia. Proteus was detected in 3 samples (7.5%) and L. grayi in 1 (2.5%) sample. Salmonela and L. monocytogenes were not detected. According to the valid regulations 9 (22.5%) samples in our investigation did not reach the adequate microbiological quality. Both, yeasts and moulds were isolated from 60% of tested cheese samples with average concentrations of 5.8 × 104cfu g1and 2.0 × 104cfu g1, respectively. The genera Geotrichum (91.9%), Moniliella (5.4%) and Aspergillus (2.7%) were the most frequently isolated strains from examined cheese samples. The Aspergillus strains did not belong to the species A. flavus or A. parasiticus and did not produce aflatoxins.

Key words: milk products / cheese / microbiology / pathogen micro-organisms / yeasts / moulds / aflatoxins
 

[1] Cfu: colony forming units


 

University of Ljubljana Biotechnical Faculty