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Acta agriculturae Slovenica, 90(November 2007)1, 5–16.

Agris category codes: Q04
COBISS Code           1.01
Language: English

RELATION OF MYOFIBRIL FRAGMENTATION TO TEXTURAL AND CHEMICAL PARAMETERS OF AGED PORK Longissimus dorsi [1]

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a), Tomaž POLAK, Božidar ŽLENDER and Lea GAŠPERLIN

a) Univ. of Ljubljana, Biotechnical Fac., Dept. of Food Science and Technology, Jamnikarjeva 101, SI-1111 Ljubljana, Slovenia

ABSTRACT

The relation of myofibrilar fragmentation (length of myofibrilar fragments, myofibrilar fragmentation index) to textural (Warner-Bratzler share force) and chemical parameters (non-protein nitrogen changes) of pork Longisimus dorsi muscles (acquired 24 h post mortem, normal meat quality) were investigated over a 16-day ageing period at 2 °C (± 1 °C). Ageing time significantly affected all parameters at the 1% level or less. The pH value for 16-day aged samples was slightly higher; the average values being 5.61 for nonaged and 5.67 for aged samples. Length of myofibrilar fragments being in average the highest (73 mm) for nonaged samples, and the lowest (15.7 mm and 12.4 mm) for 11 and 16 days aged ones. Myofibrilar fragmentation index increases significantly with storage: for nonaged samples below 30, after a 2-day ageing about 50, and after 5-day ageing 63.7 (determined as described by Olson et al., 1976), or after 11-day ageing 56.9 (determined as described by Hopkins et al., 2004) Indexes for Hopkins procedure were about 7% lower (P ≤ 0.001) compared to those for Olson procedure. Non-protein nitrogen after 11 and 16 days of storage was higher (10.78% and 10.93% of total nitrogen) compared to the nonaged pork (9.39% of total nitrogen). Warner-Bratzler share force was markedly affected by 16-day ageing (nonaged 51.3 N, 16 days 29.2 N). On the basis of instrumentally measured texture differences in thermally treated aged pork we concluded that myofibrilar fragmentation index was a suitable proteolysis rate pointer already from the second day on. The increase in non-protein nitrogen content indicates a release of free amino acids; so, it is a suitable measure of proteolysis after 5 days of ageing.

Key words: pigs / meat / ageing / myofibrilar fragments / length / myofibrilar fragmentation index / non-protein nitrogen / Warner-Bratzler shear force
 

[1] This article is part of a dissertation thesis 'Effects of quality, proteolysis and degree of doneness on heterocyclic aromatic amines formation in thermal aged pork Longissimus dorsi', issued by Dejan Došler, supervisor Assoc. Prof. Lea Gašperlin, Ph.D.


Acta agriculturae Slovenica, 90(November 2007)1, 17–24.

Agris category codes: L20
COBISS Code           1.02
Language: English

Phenotypic heterogeneity in bacterial populations

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a)

a) Univ. of Ljubljana, Biotechnical Fac., Dept. of Biology, Večna pot 111, SI-1000 Ljubljana, Slovenia, Prof., Ph.D.

ABSTRACT

Genetically uniform bacterial cells exhibit heterogeneity such as intrapopulation differences in metabolism as well as variation in growth rate. Additionally, phenotypic heterogeneity in more complex developmental processes where a portion of a population performs specialized functions has been described. Heterogeneity within populations of bacterial cells ensures that a small fraction of the population is prepared to survive adverse environmental conditions. Phenotypic heterogeneity is mediated by two mechanisms: (i) genotypic alterations such as, mutations and rearrangements of specific DNA fragments or (ii) epigenetic phenomenon. Here examples of genotypic as well as epigenetically regulated phenotypic heterogeneity from several bacterial species are presented.

Key words: microbiology / bacteria / phenotypic heterogeneity / genotype / epigenetic regulation
Acta agriculturae Slovenica, 90(November 2007)1, 25–31.

Agris category codes: L20
COBISS Code           1.01
Language: English

THE RELATIONSHIP BETWEEN TOTAL AND CULTURABLE BACTERIA IN COLD SOILS

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a)

a) Univ. of Ljubljana, Biotechnical Fac., Zootechnical Dept., Groblje 3, SI-1230 Domžale, Slovenia, Ph.D., M.Sc.

ABSTRACT

Cold ecosystems are essential to global ecology. Microbial activities are predicted to increase in low temperature regions due to global warming thus further affecting the green gas emissions and stored carbon overturn. This all has led to increased awareness how minute is our understanding of bacterial assemblages in the cold soils and also other environments, spurring the idea of standardization of research protocols. This work focused on comparison of recently published data on culturability of bacteria from 23 cold soils in the frame of International polar Year 2007 (IPY 2007). The results show that linear relationship exists between direct counts and numbers of cultured bacteria. 11 environmental parameters were reported in these studies. However, only two categories were present in all, preventing attempt to identify governing environmental factors. As there is such heterogeneity in reporting and performing research in microbial ecology, standardization of approaches and protocols in microbial ecology could improve comparability of results substantially.

Key words: microbiology / bacteria / microbial ecology / cold soil / direct count / cultivation
Acta agriculturae Slovenica, 90(November 2007)1, 33–43.

Agris category codes: F30
COBISS Code           1.01
Language: English

GENETIC VARIABILITY OF SOME QUALITY TRAITS IN Lathyrus spp. GERMPLASM

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a), Abd El-Zahar MUSTAFA, Salwa BADR and Walla TAHR

a) Tanta Univ., Fac. of Science, Botany Dept., Tanta, Egypt

ABSTRACT

Sixty-six accessions representing eighteen species of the genus Lathyrus collected from different geographic regions were evaluated for variations of quality traits (100 seeds weight, ash, total seed proteins and 3-(-N-oxayl)-L-2,3 diaminopropoinc acid – ODAP contents). High variability of ODAP levels was exhibited at both inter-specific and intra-specific levels. This variability was attributed to genetic and environmental factors. No significant correlation was found between ODAP and each of total protein content, ash content and 100 seeds weight. Cluster analysis of C.V. (Coefficient of variance) values for each accession identified the sixty-six accessions into eight groups. The most promising accession for breeding programs was L. sativus from Tunisia. This accession has good grain quality due to relatively low ODAP level and high protein content. The variations of protein content, ash content and 100-seeds weight were also discussed.

Key words: legumes / Lathyrus sativus / ODAP / genetic variability / quality traits / germplasm
Acta agriculturae Slovenica, 90(November 2007)1, 45–56.

Agris category codes: E10
COBISS Code           1.01
Language: English

optimisation of production activities on individual agricultural holdings IN THE FRAME OF DIFFERENT direct payments OPTIONS

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a), Emil ERJAVEC and Stane KAVČIČ

a) Univ. of Ljubljana, Biotehnical Fac., Dept. of Animal Science, Groblje 3, SI-1230 Domžale, Slovenia

ABSTRACT

Linear programming model has been developed and applied to the hypothetical agricultural holding in the hilly part of Slovenia in order to find optimal production plans by maximizing total gross margins. The model covers especially those sectors of Slovenian agriculture, for which the most drastic changes due to the actual reform of CAP in the field of direct payments – in Slovenia implemented in 2007 – are anticipated. On the basis of developed model the economic impacts of CAP reform and importance of agri-environmental measures have been evaluated. Model results lead to the conclusion that the reform will have the most unfavorable impacts for agricultural holdings with intensive production practice, especially those with animal production activities which are under the standard scheme eligible for relatively high production coupled direct payments (up to 70% of achieved total gross margin). Negative impacts of the reform can be mitigated by combining different production activities and technologies. Economic results markedly improve (up to 28%) if farming management complies with agri-environmental measures.

Key words: agriculture / linear programming / CAP reform / direct payments / Slovenia

 

University of Ljubljana Biotechnical Faculty