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Acta agriculturae Slovenica, Supplement 2 (September 2008), 149–154.

Agris category codes: L50, L73
COBISS Code           1.08
Language: English

THE INFLUENCE OF IMMUNOGLOBULIN STATUS ON HEALTH STATUS AND GROWTH PERFORMANCE IN CALVES TO THE AGE OF 24 WEEKS

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a), Tomaž ZADNIK, Jože STARIČ, Marija NEMEC and Jožica JEŽEK

a) Univ. of Ljubljana, Veterinary Faculty, Gerbičeva 60, SI-1000 Ljubljana, Slovenia

ABSTRACT
The aim of the research was to study the influence of immune status on health status and changing of heart girth with age in calves. Seventy one dairy calves from birth to the age of 24 weeks were studied and their health status was monitored. The blood samples were taken once a week, till the age of 6 weeks and later on at the age of 8, 12, 16, 20 and 24 weeks. In blood samples the concentration of IgG was measured with quantitative ELISA test. At each bleeding, the heart girth was measured. The average IgG concentration in the 1st week of age was 26.15 ± 15.18 g/L. The calves were divided in two groups; in the 1st group there were calves with IgG concentration in the 1st week below average value, in the 2nd one were calves with IgG concentration above average. Statistically significant differences between groups were established at the age of one (P < 0.001), two (P = 0.044), five (P = 0.001), eight (P = 0.017) and sixteen weeks (P = 0.029). The percentage of diseased calves was significantly higher in group 1 regarding to group 2. The heart girths between groups did not differ significantly. In the research it was established that the differences between groups in IgG level remained to the end of research period and more calves diseased in the low IgG group.

Key words: cattle / calves / health status / immunoglobulins / heart girth
Acta agriculturae Slovenica, Supplement 2 (September 2008), 155–162.

Agris category codes: L51
COBISS Code           1.08
Language: English

THE RELATIONSHIP BETWEEN DIET AND RETAINED PLACENTA IN COWS [1]

Krunoslav ALŠIĆ, Matija DOMAĆINOVIĆ, Željko PAVIČIĆ, Željko BUKVIĆ, Mirjana BABAN and

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a)

a) Josip Juraj Strossmayer Univ. of Osijek, Fac of Agriculture, Dept. of Animal Husbandry, Trg svetog Trojstva 3,  HR-31000 Osijek, Croatia

ABSTRACT
Retained placenta (RP) is one of the most common complications occurring in the post-partum period in cows. Beside other reasons, many authors stress the influence of diet on the incidence of RP. However, the role of minerals and vitamins fed at this time on subsequent fertility is poorly understood. As nutritional causes of RP are due primarily to the diet fed the last 6 to 8 weeks before calving, the aim of this paper was to investigate the relationship between diet in dry and transition periods and subsequent occurrence of RP, as well as in the time of introduction to lactation in fresh cows, with special emphasis on content of minerals and vitamins in diet. The investigation has been carried out on a big dairy farm situated on the east of Croatia with 1 775 Holstein-Frisian (HF) cows in duration of one year. Diet samples from dry period (DP), from period approximately from the 21st day before calving till the day of calving (BC) and period of introduction to lactation (IL) were subjected for analysis of calcium (Ca), phosphorous (P), selenium (Se), iodine (I) and vitamins A, D3 and E. The observed rate of cows with RP on the investigating farm was rather high (16.55%). High diet concentrations both for Ca and P in DP and BC periods (0.98 and 0.62% of Ca; 0.37 and 0.52% of P, respectively), combined with low concentration of vitamin D in DP and BC periods (12 800 and 14 400 IU/day, respectively), as well as low concentration of Se in DP and BC periods (0.08 and 0.07 mg/kg, respectively) combined with low concentration of vitamin E in DP and BC periods (160 and 180 IU/day, respectively) were identified as critical factors that could have contributed in influencing high incidence of RP on the investigating farm.

Key words: cattle / dairy cows / animal nutrition / minerals / vitamins / reproduction / diseases / retained placenta

[1] The article is a part of graduation thesis (justification Jun 12th, 2003), supervisor Univ. Lecturer Boris Antunović, Ph.D., D.V.M.


Acta agriculturae Slovenica, Supplement 2 (September 2008), 163–166.

Agris category codes: L50
COBISS Code           1.08
Language: English

BONE TISSUE METABOLISM IN CATTLE

Jože STARIČ, Jožica JEŽEK, Martina KLINKON, Marija NEMEC and Tomaž ZADNIK

University of Ljubljana, Veterinary faculty, Gerbičeva 60, SI-1000 Ljubljana, Slovenia

ABSTRACT
Bone metabolism is closely connected to calcium metabolism. Biochemical markers of bone metabolism in blood serum and/or urine are indicators of bone metabolic activity at real time. Disorders of bone metabolism represent a significant scope of health problems in cattle. The objective of the study was the evaluation of bone-alkaline phosphatase (BALP) a marker of bone formation and C-terminal telopeptide (CTX), a marker of bone resorption in blood serum in dairy cattle of different ages and different productive stages. The following groups of Slovenian Black and White breed cattle were investigated: calves, primiparous cows and cows in the fourth or higher lactation kept in tie-stalls type and cows at early dry off period after the fourth or higher lactation in tie-stalls type and on pasture. Calves have statistically significantly higher BALP values in blood serum than cows (P < 0.05). Statistically significantly (P < 0.05) higher values for BALP and CTX were obtained also in primiparous cows at the pick of lactation than in the fourth or higher lactation cows at the same phase of lactation. We found statistically significantly lower (P < 0.05) mean BALP blood serum activity in cows at early dry off that were housed in tie-stall type than in those on pasture. The difference in mean BALP between cows in the fourth or higher lactation at pick lactation and those at early dry off period was not statistically significant. Biomarkers of bone metabolism can be implemented in monitoring cattle bone metabolism and calcium metabolism.

Key words: cattle / bone tissue / metabolism / biomarkers
Acta agriculturae Slovenica, Supplement 2 (September 2008), 167–171.

Agris category codes: L50, L73
COBISS Code           1.08
Language: English

ANTIBODY RESPONSE FOLLOWING SOW VACCINATION USING CELL AND RECOMBINANT VACCINES, SINGLE AND MULTIPLE APPLICATION

Stane KOŠOROK a) and Miran KASTELIC

a) Farme Ihan d.d., Breznikova 89, SI-1230 Domžale, Slovenia

ABSTRACT
The vaccination of pregnant sows with E. coli antigens to increase specific antibodies in colostrum and milk and subsequently the protection of suckling piglets from neonatal E. coli diarrhea is a common and efficient method, already used for many years. The vaccination to protect individual animals against erysipelas has been used even for a longer time. There are many vaccines and vaccination techniques available on the market, among them herd specific “stable” vaccines for E. coli, inactivated cell vaccines and recombinant DNA vaccines. Unlike vaccines for pet animals, which are mostly polyvalent vaccines, there are very few polyvalent pig vaccines available on the EU market. Recently, Intervet company has developed Diluvac forte (DF) adjuvant which enables mixing of different monovalent vaccines. Porcilis DF vaccine line makes it possible to combine different antigens in the syringe and only one injection of desirable combination of antigens. The difference between two different vaccines (Colisorb, Porcilis DF) was tested to find out the antibody response following vaccination with monovalent recombinant vaccine against bivalent cell vaccine, and the antibody response followed separate and mixed injection of two antigens in one syringe. Statistical evaluation of serum antibody response obtained by ELISA test confirmed the expectations. DNA recombinant vaccines gave significantly higher antibody titers compared to cell vaccine. Porcilis vaccines mixed together prior to application gave surprisingly better response compared to separated application. The result leads to reduction of injections to pregnant sows and consequently to better immune response.

Key words: pigs / sows / immunology / antibody response / vaccination / E .coli / erysipelas

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University of Ljubljana Biotechnical Faculty